2-D DIGE reveals changes in wheat xylanase inhibitor protein families due to Fusarium graminearum DeltaTri5 infection and grain development.
Identifieur interne : 000305 ( Main/Exploration ); précédent : 000304; suivant : 0003062-D DIGE reveals changes in wheat xylanase inhibitor protein families due to Fusarium graminearum DeltaTri5 infection and grain development.
Auteurs : Emmie Dornez [Belgique] ; Evi Croes ; Kurt Gebruers ; Sebastien Carpentier ; Rony Swennen ; Kris Laukens ; Erwin Witters ; Martin Urban ; Jan A. Delcour ; Christophe M. CourtinSource :
- Proteomics [ 1615-9861 ] ; 2010.
Descripteurs français
- KwdFr :
- MESH :
- antagonistes et inhibiteurs : Xylosidases.
- microbiologie : Triticum.
- métabolisme : Protéines végétales, Triticum.
- physiologie : Fusarium.
- Protéomique, Régulation de l'expression des gènes végétaux, Électrophorèse bidimensionnelle sur gel.
English descriptors
- KwdEn :
- MESH :
- chemical , antagonists & inhibitors : Xylosidases.
- chemical , metabolism : Plant Proteins.
- metabolism : Triticum.
- microbiology : Triticum.
- physiology : Fusarium.
- Electrophoresis, Gel, Two-Dimensional, Gene Expression Regulation, Plant, Proteomics.
Abstract
Wheat contains three different classes of proteinaceous xylanase inhibitors (XIs), i.e. Triticum aestivum xylanase inhibitors (TAXIs) xylanase-inhibiting proteins (XIPs), and thaumatin-like xylanase inhibitors (TLXIs) which are believed to act as a defensive barrier against phytopathogenic attack. In the absence of relevant data in wheat kernels, we here examined the response of the different members of the XI protein population to infection with a DeltaTri5 mutant of Fusarium graminearum, the wild type of which is one of the most important wheat ear pathogens, in early developing wheat grain. Wheat ears were inoculated at anthesis, analyzed using 2-D DIGE and multivariate analysis at 5, 15, and 25 days post anthesis (DPA), and compared with control samples. Distinct abundance patterns could be distinguished for different XI forms in response to infection with F. graminearum DeltaTri5. Some (iso)forms were up-regulated, whereas others were down-regulated. This pathogen-specific regulation of proteins was mostly visible at five DPA and levelled off in the samples situated further from the inoculation point. Furthermore, it was shown that most identified TAXI- and XIP-type XI (iso)forms significantly increased in abundance from the milky (15 DPA) to the soft dough stages (25 DPA) on a per kernel basis, although the extent of increase differed greatly. Non-glycosylated XIP forms increased more strongly than their glycosylated counterparts.
DOI: 10.1002/pmic.200900493
PubMed: 20391529
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Delcour</name></author><author><name sortKey="Courtin, Christophe M" sort="Courtin, Christophe M" uniqKey="Courtin C" first="Christophe M" last="Courtin">Christophe M. Courtin</name></author></analytic><series><title level="j">Proteomics</title><idno type="eISSN">1615-9861</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Electrophoresis, Gel, Two-Dimensional (MeSH)</term><term>Fusarium (physiology)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Plant Proteins (metabolism)</term><term>Proteomics (MeSH)</term><term>Triticum (metabolism)</term><term>Triticum (microbiology)</term><term>Xylosidases (antagonists & inhibitors)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Fusarium (physiologie)</term><term>Protéines végétales (métabolisme)</term><term>Protéomique (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Triticum (microbiologie)</term><term>Triticum (métabolisme)</term><term>Xylosidases (antagonistes et inhibiteurs)</term><term>Électrophorèse bidimensionnelle sur gel (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Xylosidases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Xylosidases</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines végétales</term><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Fusarium</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fusarium</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Electrophoresis, Gel, Two-Dimensional</term><term>Gene Expression Regulation, Plant</term><term>Proteomics</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Protéomique</term><term>Régulation de l'expression des gènes végétaux</term><term>Électrophorèse bidimensionnelle sur gel</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Wheat contains three different classes of proteinaceous xylanase inhibitors (XIs), i.e. Triticum aestivum xylanase inhibitors (TAXIs) xylanase-inhibiting proteins (XIPs), and thaumatin-like xylanase inhibitors (TLXIs) which are believed to act as a defensive barrier against phytopathogenic attack. In the absence of relevant data in wheat kernels, we here examined the response of the different members of the XI protein population to infection with a DeltaTri5 mutant of Fusarium graminearum, the wild type of which is one of the most important wheat ear pathogens, in early developing wheat grain. Wheat ears were inoculated at anthesis, analyzed using 2-D DIGE and multivariate analysis at 5, 15, and 25 days post anthesis (DPA), and compared with control samples. Distinct abundance patterns could be distinguished for different XI forms in response to infection with F. graminearum DeltaTri5. Some (iso)forms were up-regulated, whereas others were down-regulated. This pathogen-specific regulation of proteins was mostly visible at five DPA and levelled off in the samples situated further from the inoculation point. Furthermore, it was shown that most identified TAXI- and XIP-type XI (iso)forms significantly increased in abundance from the milky (15 DPA) to the soft dough stages (25 DPA) on a per kernel basis, although the extent of increase differed greatly. Non-glycosylated XIP forms increased more strongly than their glycosylated counterparts.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20391529</PMID><DateCompleted><Year>2010</Year><Month>09</Month><Day>16</Day></DateCompleted><DateRevised><Year>2010</Year><Month>06</Month><Day>22</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1615-9861</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><Issue>12</Issue><PubDate><Year>2010</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Proteomics</Title><ISOAbbreviation>Proteomics</ISOAbbreviation></Journal><ArticleTitle>2-D DIGE reveals changes in wheat xylanase inhibitor protein families due to Fusarium graminearum DeltaTri5 infection and grain development.</ArticleTitle><Pagination><MedlinePgn>2303-19</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/pmic.200900493</ELocationID><Abstract><AbstractText>Wheat contains three different classes of proteinaceous xylanase inhibitors (XIs), i.e. Triticum aestivum xylanase inhibitors (TAXIs) xylanase-inhibiting proteins (XIPs), and thaumatin-like xylanase inhibitors (TLXIs) which are believed to act as a defensive barrier against phytopathogenic attack. In the absence of relevant data in wheat kernels, we here examined the response of the different members of the XI protein population to infection with a DeltaTri5 mutant of Fusarium graminearum, the wild type of which is one of the most important wheat ear pathogens, in early developing wheat grain. Wheat ears were inoculated at anthesis, analyzed using 2-D DIGE and multivariate analysis at 5, 15, and 25 days post anthesis (DPA), and compared with control samples. Distinct abundance patterns could be distinguished for different XI forms in response to infection with F. graminearum DeltaTri5. Some (iso)forms were up-regulated, whereas others were down-regulated. This pathogen-specific regulation of proteins was mostly visible at five DPA and levelled off in the samples situated further from the inoculation point. Furthermore, it was shown that most identified TAXI- and XIP-type XI (iso)forms significantly increased in abundance from the milky (15 DPA) to the soft dough stages (25 DPA) on a per kernel basis, although the extent of increase differed greatly. Non-glycosylated XIP forms increased more strongly than their glycosylated counterparts.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Dornez</LastName><ForeName>Emmie</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Department of Microbial and Molecular Systems, Katholieke Universiteit Leuven, Leuven, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Croes</LastName><ForeName>Evi</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Gebruers</LastName><ForeName>Kurt</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Carpentier</LastName><ForeName>Sebastien</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Swennen</LastName><ForeName>Rony</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Laukens</LastName><ForeName>Kris</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Witters</LastName><ForeName>Erwin</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Urban</LastName><ForeName>Martin</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Delcour</LastName><ForeName>Jan A</ForeName><Initials>JA</Initials></Author><Author ValidYN="Y"><LastName>Courtin</LastName><ForeName>Christophe M</ForeName><Initials>CM</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Proteomics</MedlineTA><NlmUniqueID>101092707</NlmUniqueID><ISSNLinking>1615-9853</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.-</RegistryNumber><NameOfSubstance UI="D014995">Xylosidases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D015180" MajorTopicYN="Y">Electrophoresis, Gel, Two-Dimensional</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005670" MajorTopicYN="N">Fusarium</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="Y">Proteomics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014908" MajorTopicYN="N">Triticum</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014995" MajorTopicYN="N">Xylosidases</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="Y">antagonists & inhibitors</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>4</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>4</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>9</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20391529</ArticleId><ArticleId IdType="doi">10.1002/pmic.200900493</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Belgique</li></country><region><li>Province du Brabant flamand</li><li>Région flamande</li></region><settlement><li>Louvain</li></settlement><orgName><li>Katholieke Universiteit Leuven</li></orgName></list><tree><noCountry><name sortKey="Carpentier, Sebastien" sort="Carpentier, Sebastien" uniqKey="Carpentier S" first="Sebastien" last="Carpentier">Sebastien Carpentier</name><name sortKey="Courtin, Christophe M" sort="Courtin, Christophe M" uniqKey="Courtin C" first="Christophe M" last="Courtin">Christophe M. Courtin</name><name sortKey="Croes, Evi" sort="Croes, Evi" uniqKey="Croes E" first="Evi" last="Croes">Evi Croes</name><name sortKey="Delcour, Jan A" sort="Delcour, Jan A" uniqKey="Delcour J" first="Jan A" last="Delcour">Jan A. Delcour</name><name sortKey="Gebruers, Kurt" sort="Gebruers, Kurt" uniqKey="Gebruers K" first="Kurt" last="Gebruers">Kurt Gebruers</name><name sortKey="Laukens, Kris" sort="Laukens, Kris" uniqKey="Laukens K" first="Kris" last="Laukens">Kris Laukens</name><name sortKey="Swennen, Rony" sort="Swennen, Rony" uniqKey="Swennen R" first="Rony" last="Swennen">Rony Swennen</name><name sortKey="Urban, Martin" sort="Urban, Martin" uniqKey="Urban M" first="Martin" last="Urban">Martin Urban</name><name sortKey="Witters, Erwin" sort="Witters, Erwin" uniqKey="Witters E" first="Erwin" last="Witters">Erwin Witters</name></noCountry><country name="Belgique"><region name="Région flamande"><name sortKey="Dornez, Emmie" sort="Dornez, Emmie" uniqKey="Dornez E" first="Emmie" last="Dornez">Emmie Dornez</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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